ANTI-ADAM 9 ANTIBODY DISINTEGRIN DOMAIN (C15-1315-991)

General description

A disintegrin and metalloproteinase domain-containing protein 9 (ADAM 9) belongs to a family of transmembrane, membrane-anchored, disintegrin-containing proteins that are involved in several biological functions such as protein ectodomain shedding, cell fusion, and cell adhesion. ADAMs contain several characteristic domains, two of which, the protease and disintegrin domains, are noted for their homology to snake venom metalloprotease. The exact function of ADAM 9 is yet unknown, however it is widely distributed, is found within keratinocytes, and is ideally placed on the cell membrane, which some studies have suggested supports a role in cell migration and motility. Alternative splicing results in two isoforms; isoform 1 is found within the cell membrane and is considered a single-pass type 1 membrane protein, while isoform 2 is secreted. Mutations in ADAM 9 expression are causal to cone-rod dystrophy type 9 or CORD9 which is characterized by visible retinal pigment deposits and loss of cone photoreceptors leading and rod degeneration, ultimately resulting in severe loss of vision.

Specificity

Mouse and rat (90% sequence homology).

Immunogen

Epitope: disintegrin domain

KLH-conjugated linear peptide corresponding to the disintegrin domain of human ADAM 9.

Application

Detect ADAM 9 using this Anti-ADAM 9 Antibody, disintegrin domain validated for use in WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Quality

Evaluated by Western Blot in human chondrocyte cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected ADAM 9 in 10 µg of human chondrocyte cell lysate.

Target description

~ 90 kDa observed. 2 isoforms are produced by alternative splicing: Isoform 1 at 91 kDa and Isoform 2 at 72 kDa

Linkage

Replaces: AB19025

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human chondrocyte cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology), primate (based on 100% sequence homology). technique(s): western blot: suitable. NCBI accession no.: NP_003807. UniProt accession no.: Q13443. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... ADAM12(8038), ADAM9(8754). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.