Anti-Aggrecan Antibody (C15-1315-632)

General description

Aggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight.

Specificity

AB1031 is specific for mouse aggrecan; other species have not been tested.

Expected to cross react with Human and rat.

Immunogen

GST fusion protein containing amino acids 1177-1326 of mouse aggrecan.

Application

Anti-Aggrecan Antibody is an antibody against Aggrecan for use in IF, IH(P), IP & WB.

Immunohistochemistry(Paraffin):
A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments.

Immunoprecipitation:
A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates.

Physical form

ImmunoAffinity Purified

Rabbit polyclonal antigen affinity purified antibody in PBS. Anti-GST has not been adsorbed. Liquid.
Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Cartilage, neural tube, and brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse. manufacturer/tradename: Chemicon^®. . technique(s): immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_001126. UniProt accession no.: P16112. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... ACAN(176). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.