Anti-ALKBH3 antibody produced in rabbit (C15-1447-333)

General description

ALKBH3 (alkB homolog 3, α-ketoglutarate-dependent dioxygenase), also known as prostate cancer antigen (PCA)1, shows high homology to E. coli alkB protein. It is a DNA and RNA repair enzyme.

Immunogen

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

ALKBH3 (alkB homolog 3, α-ketoglutarate-dependent dioxygenase) is overexpressed in non-small-cell lung cancer and results in increased cell proliferation. Inactivation of this protein leads to the activation of p21 and p27, which causes cell cycle arrest. It interacts with NOX (nitric oxide)-2-ROS (reactive oxygen species) and Tweak (TNF homologue with weak apoptosis-inducing activity)/Fn14 (fibroblast growth factor-inducible protein 14)-VEGF (vascular endothelial growth factor) signaling pathways and promotes survival, angiogenesis, and invasion in urothelial carcinomas. It has potential as a diagnostic marker for prostate cancer (PC), and might be one of the more aggressive variants of PC. It promotes tumorigenesis in pancreatic cancer by facilitating angiogenesis and resistance to apoptosis.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST71083.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.