Anti-alpha-Actin Mouse mAb (1A4)

General description

Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.

Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).

This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

Immunogen

Human Smooth Muscle

a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

Application

Frozen Sections (1:50-1:75)

Immunoblotting (see comments)

Paraffin Sections (1:50-1:75)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

Analysis Note

Negative Control
Fibroblasts, striated muscle, or myocardium

Positive Control
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary

Other Notes

Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.

Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

Legal Information

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