ANTI-ALPHA DEFENSIN-5 (HD5) ANTIBODY CLONE 8C8 (C005B-450097)

General description

Interleukin-1 beta (UniProt: P01584; also known as IL-1 beta, Catabolin) is encoded by the IL1B (also known as IL1F2) gene (Gene ID: 3553) in human. IL-1β is one of the best characterized potent pro-inflammatory cytokines that is critical for host-defense responses to infection and injury. It is produced and secreted by a variety of cell types; however, it is mainly expressed in activated monocytes/macrophages. IL-1β is produced as pro-IL-1β in response to molecular motifs carried by pathogens known as pathogen associated molecular patterns (PAMPs). Its synthesis occurs in two steps and each step is controlled by different stimuli. In the initial step, inflammatory signals, such as lipopolysaccharides, stimulate the synthesis and promote the accumulation of pro-IL-1β in cytoplasm. Following this step pro-IL-1β is cleaved by the caspase-1, the activation of which occurs via recruitment to inflammasome. This step removes the propeptide (1-116) segment. Following caspase-1-dependent processing of pro-IL-1β, mature IL-1β (aa 117-269) is rapidly secreted from cells. IL-1β is known to induce prostaglandin synthesis, neutrophil influx and activation, T-cell and B-cell activation and antibody production. It also promotes Th17 differentiation and synergizes with IL-12 to induce interferon g from T-helper cells. (Ref.: Lopez-Castejon, G., and Brough, D. (2011). Cytokine Growth Factor Rev. 22(4); 189-195; Tominaga, K., et al. (2000). Int. Immunol. 12(2); 151-160).

Specificity

Clone 11G7.3.7 is a mouse monoclonal antibody that detects human Interleukin-1β (IL-1β)

Immunogen

Full-length mature recombinant human Interleukin-1β (without the propeptide).

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant human Interleukin-1β (IL-1β).

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected human recombinant Interleukin-1β (IL-1β).

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-1β in human lung tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-1β, clone 11G7.3.7, Cat. No. MABF3183, is a mouse monoclonal antibody that detects Interleukin-1β (IL-1β) and is tested for use in Immunohistochemistry (Paraffin) and in Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2b in PBS with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.