ANTI-ALPHA DEFENSIN-5 (HD5) ANTIBODY CLONE 8C8 (C005B-450106)

General description

Interleukin-6 (UniProt: P05231; also known as IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2) is encoded by the IL6 (also known as IFNB2) gene (Gene ID: 3569) in human. IL-6 is an immunoregulatory secreted cytokine that is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off to produce the mature form (aa 30-212). It is mainly produced in skeletal muscle and its levels are shown to increase upon exercise due to enhanced production by contracting skeletal muscles. It is a potent inducer of the acute phase response and rapid IL-6 production contributes to host defense during infection and tissue injury. In the innate immune response, it is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection. In the adaptive immune response, it is required for the differentiation of B cells into immunoglobulin-secreting cells. It is reported to plays a major role in the differentiation of CD4+ T cell subsets and is an essential factor for the development of T follicular helper (Tfh) cells. Its actions are mediated by binding to IL-6 receptor (IL-6R) and the ligand-receptor complex associates to the signaling subunit IL6ST/gp130. The interaction with the membrane-bound IL6R and IL6ST is reported to stimulate the classic signaling, whereas the binding of IL6 and soluble IL6R to IL6ST stimulates trans-signaling where synergistically with IL-1b and TNF it can induce the production of VEGF and stimulate angiogenesis. (Ref.: Nakahara, H., et al. (2003). Arthritis Rheum. 48(6); 1521-1529; Boulanger, MJ., et al. (2003). Science. 300(5628); 2101-2104).

Specificity

Clone 3A7.10 is a mouse monoclonal antibody that detects human IL-6.

Immunogen

Recombinant fragment of human Interleukin 6 (IL-6) corresponding to amino acids 29-212.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant human IL-6.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-6.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-6 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-6, clone 3A7.10, Cat. No. MABF3179, is a mouse monoclonal antibody that detects human Interleukin-6 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.