General description
The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.
Specificity
This antibody recognizes α-Insulin Receptor, β subunit.
Immunogen
Epitope: C-terminus
GST-fusion protein corresponding to the last 100 C-terminal amino acid residues of the human insulin receptor β chain
Application
Anti-α-Insulin Receptor Antibody, β subunit is an antibody against a-Insulin Receptor for use in IP & WB.
Immunoprecipitation Analysis:
Research Category
Signaling
Metabolism
Research Sub Category
Insulin/Energy Signaling
Quality
Evaluated by Western Blot in Human brain tissue lysate.
Western Blot Analysis: : 1:1,000 dilution of this antibody detected α-Insulin Receptor, β subunit in human brain tissue lysate.
Target description
97 kDa
Physical form
Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
Storage and Stability
Maintain refrigerated at 2-8°C for for 1 year from date of receipt.
Analysis Note
Control
Human brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51183616
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 07-724
- Temperature Control Device:
- Yes
