General description

The synucleins are a family of soluble presynaptic proteins that are abundant in neurons and include α-synuclein, β-synuclein, and γ-synuclein. Human α-synuclein (also known as the non-amyloid component of plaques precursor protein or NACP) is a 140-amino acid polypeptide that is encoded by a gene on chromosome 4. It was originally isolated from plaques of Alzheimerâ€²s disease (AD) brains as a 19 kDa protein precursor of the highly hydrophobic 35-amino acid peptide, nonamyloid component (NAC) of plaques. The NAC can self-aggregate into fibrils and induce aggregation of the β-amyloid peptide. α-Synuclein is highly abundant in presynaptic terminals, and is a major component of Lewy bodies (LBs). Pathogenic point mutations in the α-synuclein gene are linked to familial Parkinsonâ€²s disease (PD) in rare kindreds. However, most neurodegenerative disorders with LBs are associated with abnormal accumulation of wild-type, α-synuclein. α-Synuclein is expressed primarily in brain, but is also expressed in low levels in all tissues examined except liver.

Specificity

Rabbit polyclonal anti-α-Synuclein antibody recognizes human and rat α-synuclein. Staining of α-synuclein in immunoblotting is specifically inhibited by the immunizing peptide

Immunogen

synthetic peptide corresponding to a sequence near the C-terminus of human α-synuclein (amino acids 111-132 with C-terminally added lysine) conjugated to KLH. This sequence is highly conserved (90% homology) in mouse and rat α-synuclein. This sequence has no homology with β- and γ-synuclein.

Application

Rabbit polyclonal anti-α-Synuclein antibody is used to tag α-Synuclein for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques, such as immunoblotting. It is also used as a probe to determine the presence and roles of α-Synuclein in plaques of Alzheimerâ€²s disease (AD) brains.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

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