Anti-alpha-Synuclein (C15-1317-656)

General description

Alpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is abundantly expressed in the brain and is found in a classic amyloid fibril form within the intra-neuronal Lewy body deposits of Parkinson′s disease brains. The physiological function of synucleins is not well understood, but appears to involve membrane interactions, and in particular reversible binding to synaptic vesicle membranes. It may also be involved in the regulation of dopamine release and transport. It is also reported to reduce neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Genetic alterations of SNCA gene is reported to result in aberrant polymerization into fibrils, which is associated with several neurodegenerative diseases (synucleinopathies). Mutations in SNCA gene are also known to Parkinson′s disease characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology of Parkinson s disease involves progressive loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies in surviving neurons in various areas of the brain.

Specificity

This rabbit polyclonal antibody detects alpha synuclein in human, mouse, and rat. It targets an epitope with in 21 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Alpha-Synuclein.

Application

Anti-alpha-Synuclein, Cat. No. ABN905, is a rabbit polyclonal antibodt that detects alpha synuclein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: A 1:1,000-2,000 dilution from a representative lot detected alpha-Synuclein in human and rat brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:2,000-3,000 dilution from a representative lot detected alpha-Synuclein in human cerebral cortex and rat cerebellum tissue sections.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected alpha-Synuclein in mouse brain tissue lysate.

Target description

~17 kDa observed; 14.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Other Notes

Concentration: Please refer to lot specific datasheet.

biological source: rabbit. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse, rat. packaging: antibody small pack of 25 . μ. g. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NM_000345.3. UniProt accession no.: P37840. target post-translational modification: unmodified. Gene Information: human ... SNCA(6622).