General description
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Immunogen
A peptide corresponding to the C-terminus of α-Tubulin.
Application
Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected alpha-Tubulin in A431 cells.
This Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected alpha-Tubulin in A431 cell lysate.
Target description
~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
Shipping Information:
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- UPC:
- 41106514
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABT522
- Temperature Control Device:
- Yes
