ANTI-AMELOGENIN X ISOFORM/AMELX ANTIBODY (C005B-241835)

General description

Amelogenin, X isoform/AMELX, also known as Leucine-rich Amelogenin peptide, or LRAP, and encoded by the gene AMELX/AMG/AMGX, is an important protein in the biomineralization of teeth. AMELX appears to regulate the formation of crystallites during the secretory stage of tooth enamel development. AMELX is a secreted extracellular matrix protein that has many isoforms. Interestingly mutations in the AMELX protein are associated with changes in enamel microstructure that are linked with severe dental disease, and the various isoforms of AMELX protein all aid in increasing enamel thickness of the developing tooth.

Immunogen

Recombinant protein corresponding to mouse Amelogenin, X isoform/AMELX.

Application

Anti-Amelogenin, X isoform/AMELX Antibody is an antibody against Amelogenin for use in western blotting & IHC.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Amelogenin, X isoform/AMELX using recombinant mouse Amelogenin179.

Western Blotting Analysis: A representative lot from an independent laboratory detected Amelogenin, X isoform/AMELX in DESC spheres and cervical loop lysates (Chang, J. Y., et al. (2013). J Cell Biol. 288(40):28952-289561.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Amelogenin, X isoform/AMELX in DESC spheres and cervical loops (Chang, J. Y., et al. (2013). J Cell Biol. 288(40):28952-289561.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Amelogenin, X isoform/AMELX in normal ameloblasts in the wild type and the pseudostratified layer and amorphous matrix of the null mice (Rios, H., et al. (2005). Mol Cell. Biol. 25(24):11131-11144.).

Quality

Evaluated by Western Blotting in pig enamel acid extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Amelogenin, X isoform/AMELX in pig enamel acid extract.

Target description

~23 kDa observed. At least 3 isoforms may be observed between 19-23 kDa in pig enamel acid extracts (Chang, J. Y., et al. (2013). J Cell Biol. 288(40):28952-289561.). Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: porcine, mouse. technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_033796. UniProt accession no.: P63277. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Amelx(11704). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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