Specificity
Recognizes a glycoprotein of 18.5-22.5kDa, identified as amphiregulin (AR). Antibody is directed against the region of AR, which binds to heparin and is highly specific to AR. It shows no cross-reaction with EGF, TGFalpha, or HB-EGF. In vitro, AR functions as an autocrine growth stimulator to derive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. AR is important in development of human placenta and murine mammary gland.
Immunogen
A synthetic 19-mer peptide, corresponding to aa 26-44 from the secreted amphiregulin of human origin.
Application
Immunoprecipitation (Use Protein A): Ab 10μg/mg protein lysate.
Western Blotting: Ab 5μg/ml for 2hrs at room temperature.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
This Anti-Amphiregulin Antibody is validated for use in ELISA, IP, WB for the detection of Amphiregulin.
Physical form
Format: Purified
Purified immunoglobulin from rabbit anti-serum by Protein A chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide.
Storage and Stability
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, mouse. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NM_001657.2. UniProt accession no.: P15514. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... AREG(374). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51201516
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB3152
