Anti-Amyloid Antibody, beta 1-40/42 (C15-1316-179)

Specificity

Recognizes beta-amyloid 1-40/42. One of the most important and initial steps which causes loss of memory and cognition in Alzheimer′s Disease (AD) involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 amino acid peptides (beta amyloid 1-40, 1-42 and 1-43). Polymerization of beta-amyloid and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. The immunogen peptide shows homology with beta-amyloid 1-28 and beta-amyloid 12-28. No cross reactivity is observed with CGRP.

Immunogen

A synthetic beta-amyloid peptide 1-40 conjugated to BSA.

Application

Anti-Amyloid Antibody, β 1-40/42 is an antibody against Amyloid for use in ELISA, IH & WB.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Western blot: 1:1,000-1:5,000 (Chemiluminescence technique)

Immunohistochemistry: 1:100 using formalin or paraformaldehyde fixed Alzheimer′s brain tissue.

ELISA: 1:10,000-1:100,000 (50-100 ng immunogen peptide /well).

Immunoge control peptide is Catalog number AG365.

Optimal working dilutions must be determined by the end user.

Linkage

Replaces: AB5408

Physical form

Rabbit Serum. Liquid and 0.1% sodium azide.

Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Alzheimer′s disease brain tissue, whole tissue extracts from mouse brain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, mouse. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_000484.2, NM_201413.1, NM_201414.1. UniProt accession no.: P05067. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... APP(351). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.