ANTI-AMYLOID-LIKE PROTEIN 1 ANTIBODY (C005B-241400)

General description

Amyloid-like protein 1 or APLP-1 is neuronal, membrane bound specific protein that likely has a role in post-synaptic function but most research has focused on its proteolytic fragments such as ALID1 which acts as a transcriptional activator through binding the co-activator APBB1. APLP-1 fragments also interact with various G-protein coupled receptor pathways and appear to have a role in synaptic maturation during cortical development. APLP-1 also appears to regulate neurite outgrowth through binding to components of the ECM such as heparin and collagen I. Finally a special fragment called C30 is a potent enhancer of neuronal apoptosis and is a caspase specific cleavage fragment that works internally inducing apoptosis. Thus APLP-1 has roles in apoptosis, cell adhesion, endocytosis, cell signaling and neurite extension as well as some post-synaptic function as it is found clustered at post-synaptic densities in normal cells.

Specificity

Immunogen is 94% identical to Bovine

Immunogen

Epitope: Extracellular domain

KLH-conjugated linear peptide corresponding to the extracellular domain of Human Amyloid-like protein 1.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Amyloid-like protein 1 in human cerebral cortex and thalamic tissues.

Research Category
Neuroscience

Neuroscience

Research Sub Category
Neurodegenerative Diseases

Synapse & Synaptic Biology

This Anti-Amyloid-like protein 1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Amyloid-like protein 1.

Quality

Evaluated by Western Blotting in Human Cortex tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Amyloid-like protein 1 in 10 µg of Human Cortex tissue lysate.

Target description

~72 kDa observed. A ~90kDa band may appear in some lysates due to glycosylation: Kim. TW., et al. (1995). Brain Res Mol Brain Res. 32(1):36-44.

Physical form

Antigen Affinity Purified

Purified Rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): rat (based on 100% sequence homology), mouse (based on 100% sequence homology). technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_005157. UniProt accession no.: P51693. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... APLP1(333). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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