General description
Discovered on gastrointestinal stromal tumors protein 1 (Dog1), also known as Anoctamin-1/TMEM16A, is a novel multi-pass membrane protein belonging to the anoctamin family. Anoctamin-1/TMEM16A functions as a Ca2+-activated Cl- channel and is a required component of tracheal development. Expression of Anoctamin-1/TMEM16A is elevated in the gastrointestinal stromal tumors (GISTs), KIT signaling-driven mesenchymal tumors of the GI tract. It is also expressed at more moderate levels in epithelia from foregut, kidney, lung, pancreas and salivary glands. Recent studies suggest that it may serve a roll as a diagnostic marker in the classification of GISTs.
Specificity
This antibody recognizes isoforms 1 and 2 of Anoctamin-1 based upon immunogen design.
Immunogen
Epitope: N-terminal cytoplasmic domain
GST-tagged recombinant protein corresponding to the N-terminal cytoplasmic domain of human Anoctamin-1/TMEM16A.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Anoctamin-1/TMEM16A in human prostate cancer sections.
This Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1 is validated for use in western blotting & IHC for the detection of Anoctamin-1/TMEM16A.
Quality
Evaluated by Western Blotting in PC-3 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Anoctamin-1/TMEM16A in 10 µg of PC-3 cell lysate.
Target description
~114 kDa and ~98 kDa observed. Three isoforms are known to exist at 114 kDa (isoform 1), 98 kDa (isoform 2), and 74 kDa (isoform 3).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Shipping Information:
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- UPC:
- 51171519
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABT379
- Temperature Control Device:
- Yes
