ANTI-APP CLONE 1D1

General description

Amyloid beta A4 protein (UniProt: P05067; also known as ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. APP can also be cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. In addition to its obvious role in Alzheimer′s disease, the most-substantiated role for APP is in synaptic formation and repair. Its expression is upregulated during neuronal differentiation and after neural injury. Clone 1D1 is shown to bind to the N-terminus of APP and detects both the soluble full length and secreted hAPP and can also detect transgenic APP expression in APP-transgenic animal models.

Specificity

Clone 1D1 specifically detects human amyloid precursor protein (APP) and does not display reactivity with other species. It targets an epitope within the ectodomain region of APP and does not react with A beta peptide.

Immunogen

StrepII-tagged human recombinant ectodomain region of the neuronal isoform of hAPP695 lacking the KPI domain.

Application

Anti-APP, clone 1D1, Cat. No. MABN2287, is a highly specific rat monoclonal antibody that targets Amyloid beta A4 protein and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Note: For the Western blotting application use of nitocellulose is highly recommended and the samples must be boiled in non-reduing laemmli buffer. Please note that clone 1D1 recognizes APP only in its quasi-native folded form.

Immunoprecipitation Analysis: A representative lot detected APP in Immunoprecipitation applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Western Blotting Analysis: A representative lot detected APP in WT, but not in APP knockdown HEK293T cells (Courtesy of Dr. med. Peer-Hendrik Kuhn, Ph.D., Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Munich, Germany).

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected APP in HEK293 cell line.

Immunocytochemistry Analysis: A representative lot detected APP in Immunocytochemistry applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected APP in HEK293T cells, but not in cells lentivirally transduced with APP shRNA-1 or APP shRNA-2, both coexpressing GFP as a reporter (Courtesy of Dr. med. Peer-Hendrik Kuhn, Ph.D., Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Munich, Germany).

Immunohistochemistry Analysis: A representative lot detected APP in Immunohistochemistry applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected APP in human cerebral cortex and human Alzheimer′s brain tissues.

ELISA Analysis: A representative lot detected APP in ELISA applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Western Blotting Analysis: A representative lot detected APP in Western Blotting applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Flow Cytometry Analysis: A representative lot detected APP in Flow Cytometry applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected APP in 10 µg of HEK293 cell lysate.

Target description

~100 kDa observed; 86.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.