ANTI-AQUAPORIN 4 ANTIBODY CT (C005B-239943)

General description

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.

Specificity

Reactivity with other species has not yet been tested. It is expected that the antibody may also react with bovine (71/75) and rabbit (64/75) due to sequence homology.

Recognizes Aquaporin 4 (AQP4, WCH4, Mercurial-Insensitive Water Channel).

Immunogen

Epitope: C-terminus

GST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863).

Application

Detect Aquaporin 4 using this Anti-Aquaporin 4 Antibody, C-terminus validated for use in IH & WB.

Immunohistochemistry:
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).

Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.

Optimal working dilutions must be determined by the end user

Research Category
Neuroscience

Research Sub Category
Ion & Transport Channels

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.

Target description

38 kDa

Physical form

Format: Purified

ImmunoAffinity Purified: Amber vial of Rb X Aquaporin 4, C-Terminus (part # AB3594-50ULa)

Purified rabbit polyclonal, lyophilized from buffer containing PBS, pH 7.4, containing 1% BSA, and 0.05% sodium azide. Reconstitute with 200 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Storage and Stability

Stable for up to 6 months at -20ºC from date of receipt. After reconstitution stable at -20ºC in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Clear vial of RbX Aquaporin 4, CT Control Antigen(AB3594-50ULb) included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human, rat. manufacturer/tradename: Chemicon^®. . technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_001650.4. UniProt accession no.: P55087. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... AQP4(361). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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