General description
Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ELISA, Western blotting, immunofluorescence, and immunohistochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa AQP5 protein.
Recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts.
Immunogen
a synthetic peptide corresponding to a 17-amino acid sequence in the cytoplasmic region of rat AQP5
Application
ELISA (0.5-1.0 µg/ml)
Immunoblotting (1-10 µg/ml)
Immunofluorescence (2-20 µg/ml)
Immunohistochemistry (2-20 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Analysis Note
Positive Control
Epithelial cells
Other Notes
Does not cross-react with other AQP proteins. The use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands. Variables associated with assay conditions will dictate the optimal working dilution.
Lee, M.D., et al. 1996. J. Biol. Chem. 271, 8599.
Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51283109
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 178615-50UG