ANTI-AQUAPORIN 6 ANTIBODY KIDNEY SPECIFIC FORM (C005B-239845)

General description

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Aquaporin-6 (WCH3 or hKID or AQP2-like; 282 aa; 29 kDa; chromosome 12q13) is found only in the kidney with low water permeability. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. Aquaporin-6 shows greatest homology with hMIP (48%) and hAQP-2 (52%). It also has similarity with human MIWC (AQP4; 34%), CHIP-28 (AQP1; 38%), and GLIP (AQP3; 22%).

Specificity

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Aquaporin-6 (WCH3 or hKID or AQP2-like; 282 aa; 29 kDa; chromosome 12q13) is found only in the kidney with low water permeability. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. Aquaporin-6 shows greatest homology with hMIP (48%) and hAQP-2 (52%). It also has similarity with human MIWC (AQP4; 34%), CHIP-28 (AQP1; 38%), and GLIP (AQP3; 22%)

Immunogen

A 19 AA synthetic peptide within the carboxy terminal domain of human AQP6 (Ma et al. 1996) was selected for antibody production. This domain is predicted to be cytoplasmic.

Epitope: kidney specific form

Application

Anti-Aquaporin 6 Antibody, kidney specific form is an antibody against Aquaporin 6 for use in ELISA & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1-10 μg/mL using Chemiluminescence technique Immunohistochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues.

ELISA: 0.5-1.0 μg/mL

Optimal working dilutions must be determined by end user.

Physical form

Affinity Purified immunoglobulin in PBS containing 0.1% BSA as stabilizer. No preservative.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, western blot: suitable. NCBI accession no.: NM_001652.3. UniProt accession no.: Q13520. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... AQP6(363). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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