Anti-Ars2 Antibody (C15-1317-072)

General description

As a component of the nuclear RNA cap-binding complex (CBC), Ars2 is known to play a critical role in miRNA biogenesis by stabilizing and linking capped primary miRNA transcripts with the primary miRNA processing complex containing DROSHA and DGGR8. Unlike other CBC components, Ars2 expression also plays an important role in cell proliferation. In particular, depletion of Ars2 is shown to reduce the levels of several miRNAs, such as miR-21, let-7, and miR-155, which are implicated in cellular transformation.

Immunogen

Linear peptide corresponding to human Ars2.

Application

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Ars2 in IP. (Alex Achille, Department of Molecular Oncology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, Florida, USA).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-Ars2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect Ars2 also known as Serrate RNA effector molecule homolog, Arsenite-resistance protein 2.

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Ars2 in 10 µg of HEK293 cell lysate.

Target description

~120 kDa observed. Uncharacterized bands at ~78 kDa and 48 kDa may be observed in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, mouse, human. technique(s): immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_001122324. UniProt accession no.: Q9BXP5. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SRRT(51593). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.