ANTI-ASXL2 CLONE 1D5 ZOOMAB RABBIT MONOCLONAL (C005B-262451)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D5 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Putative Polycomb group protein ASXL2.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human Putative Polycomb group protein ASXL2.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human testis tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected ASXL2 in human testis tissue sections.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ASXL2 in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound ASXL2 peptide with a KD of 1.3 x 10-9 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ASXL2 in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Immunohistochemistry (Paraffin) in human testis tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected ASXL2 in human testis tissue sections.

Target description

Putative Polycomb group protein ASXL2 (UniProt: Q76L83; also known as Additional sex combs-like protein 2) is encoded by the ASXL2 (also known as ASXH2, KIAA1685) gene (Gene ID: 55252) in human. ASXL2 is a putative Polycomb group (PcG) protein that acts by forming multiprotein complexes that are required to maintain the transcriptionally repressive state of homeotic genes throughout development. It is not required to initiate repression but is essential to maintain it during later stages of development. ASXL2 is reported to be involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as peroxisome proliferator-activated receptor (PPARG). It contains two Leu-Xaa-Xaa-Leu-Leu (LXXLL; aa 303-307 and 887-891) motifs that are required for its association with nuclear receptors. It also acts as a coactivator for PPARG and enhances its adipocyte differentiation-inducing activity; the function seems to involve differential recruitment of acetylated and methylated histone H3. ASXL2 is reported to be essential for hematopoiesis and acts as a haplo-insufficient tumor suppressor in leukemia. ASXL2 gene is one of most commonly mutated genes in AML patients bearing the AML1-ETO fusion oncoprotein. Loss of ASXL2 is shown to promote AML1-ETO-mediated leukemogenesis. Two isoforms of ASXL2 are reported that are produced by alternative splicing. Mutation in ASXL2 gene have also been linked to the development of Shashi-Pena syndrome that is characterized by delayed psychomotor development, intellectual disability, and macrocephaly, and neonatal feeding difficulties. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Micol, JB., et al. (2017). Nat. Commun. 8; 15429; Park, UH., et al. (2011). J. Biol. Chem. 286(2); 1354-1363).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.