Anti-ATF5 (C15-1317-057)

General description

Cyclic AMP-dependent transcription factor ATF-5 (UniProt: also known as cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx) is encoded by the ATF5 (also known as ATFX) gene (Gene ID: 22809) in human. ATF5 belongs to the ATF/CREB family of transcription factors that is widely expressed. It has been implicated in multiple biological processes, such as regulation of cell proliferation and differentiation, cell stress response and survival. ATF5 can either stimulate or repress gene transcription through binding of different DNA regulatory elements such as CRE (cAMP response element), ARE (ATF5 regulatory element), and AARE (amino acid response element). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. ATF5 is an essential structural protein that is required for the interaction between the mother centriole and the pericentriolar material (PCM). ATF5 depletion is reported to block PCM accumulation at the centrosome and cause fragmentation of centrioles, which contributes to the formation of multi-polar mitotic spindles and genomic instability. ATF5 homeostasis is essential for the function of the centrosome and for the global microtubule organization.

Specificity

This polyclonal antibody targets a sequence in the N-terminal region and detects ATF5 by immunocytochemstry.

Immunogen

A linear peptide corresponding to 18 amino acids from the N-terminal half of human Cyclic AMP-dependent transcription factor ATF-5.

Epitope: unknown

Application

Anti-ATF5, Cat. No. ABE2614, is a highly specific rabbit polyclonal antibody that targets Cyclic AMP-dependent transcription factor ATF-5 and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected ATF5 in 10 µg of C6 cell lysate.

Western Blotting Analysis: A representative lot detected ATF5 in Western Blot applications. (Madarampalli, B., et. al. (2015). Cell. 162(3):580-92).

Immunofluorescence Analysis: A representative lot detected ATF5 in Immunofluorescence applications. (Madarampalli, B., et. al. (2015). Cell. 162(3):580-92).

Western Blotting Analysis: A representative lot detected ATF5 in transfected Hela Cells (Courtesy of Dr. David X. Liu from Washington State University).

Immunofluorescence Analysis: A representative lot detected ATF5 in Cos-7, Hela, and C6 cells (Courtesy of Dr. David X. Liu from Washington State University).

Quality

Evaluated by Immunocytochemistry in COS-7 cells.

Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected ATF5 in COS-7 cells.

Target description

~32 kDa observed; 30.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: monkey, rat, human. technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable. NCBI accession no.: NP_036200. UniProt accession no.: Q9Y2D1. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... ATF5(22809). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.