ANTI-ATG8 CLONE 2B11 ZOOMAB RABBIT MONOCLONAL (C005B-261606)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 2B11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Drosophila ATG8. It targets an epitope within 16 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptides corresponding to 16 amino acids each from the N-terminal region of Drosophila ATG8a and ATG8b.

Application

Anti-ATG8, clone 2B11 ZooMAb, Cat. No. ZRB1585, is a recombinant Rabbit monoclonal antibody that specifically targets ATG8 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ATG8 in DSL2 Drosophila celld treated with chloroquine (50 mM overnight).

Affinity Binding Assay: A representative lot of this antibody bound ATG8 peptide with a KD of 6.0 x 10-9 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Autophagy-related 8a, isoform A and (UniProt: Q9W2S2; also known as LD05816p) and Autophagy-related 8b, isoform A (UniProt: Q9VEG5; also known as Autophagy-related 8b, isoform B, GEO01803p1) are encoded by Atg8a (also known as ATG8, DrAtg8a, LC3) and Atg8b (also known as ATG8, Atg8B) genes (Gene ID: 32001 and 42132) in Drosophila Melanogaster. Atg8 is a highly conserved and ubiquitously expressed protein that is upregulated under various stress conditions. Atg8 genes of multicellular animals is divided into three subfamilies LC3, GABARAP, GATE16 (depending on the animal). LC3 is the mammalian ortholog of Drosophila Atg8. Atg8 It shares a strong structural similarity to ubiquitin, despite the lack of similarity in amino acid sequence. The ubiquitin core of the Atg8 consists of a four-stranded central beta sheet, where the two central strands are parallel to each other and the two outer strands are antiparallel to the central strands. Between these strands are two alpha helices with alpha3 between beta2 and beta3 and alpha4 between beta3 and beta4. In resting cells, the majority of Atg8 is in found in an unlipidated state (Atg8-I) and during autophagy it is conjugated with a lipid moiety (Atg8-II). The lipidated form is the one that is recruited to autophagosomal membranes. The level of Atg8-II is an indicator of autophagosome activation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shpilka, T., et al. (2011). Genome Biology. 12(7); 226).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.