Anti-ATM antibody,Mouse monoclonal (C15-1314-873)

General description

Monoclonal Anti-ATM (mouse IgG1 isotype) is derived from the hybridoma MAT3-4G10/8 produced by the fusion of mouse myeloma cells (NSO) and splenocytes from BALB/c mice immunized with a peptide.

Specificity

Monoclonal Anti-ATM antibody reacts specifically with mouse and human ATM.

Immunogen

peptide spanning positions 1967-1988 of mouse ATM containing a cysteine at its NH2 terminus coupled to KLH.

Application

Monoclonal Anti-ATM antibody can be used in immunoblotting , ELISA and immunoprecipitation.

Biochem/physiol Actions

Anti-Ataxia-Telangiectasia Mutated (ATM) is responsible for the activation and stabilization of p53 in response to double-strand break (DSB). ATM phosphorylates p53 directly on Ser15 and concomitantly activates other kinases that phosphorylate the same molecule on additional sites. Furthermore, human homolog of double minute 2 (Hdm2) is phosphorylated by ATM on Ser395 and this phosphorylation inhibits Hdm2-mediated degradation of p53.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: mouse. Quality Level: 200. recombinant: expressed in mouse cell line. conjugate: unconjugated. antibody form: purified from hybridoma cell culture. antibody product type: primary antibodies. clone: MAT3-4G10/8, monoclonal. form: PBS solution. mol wt: antigen ~300 . kDa. species reactivity: human, mouse. packaging: antibody small pack of 25 . μ. L. concentration: ~2 . mg/mL. technique(s): immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.1-0.2 . μ. g/mL using HEK-293T total cel extract. isotype: IgG1. UniProt accession no.: Q13315. shipped in: dry ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... ATM(472)
mouse ... Atm(11920). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.