Anti-Atrial Natriuretic peptide (C15-1316-277)

General description

Natriuretic peptides A (UniProt: P01161; also known as CDD-ANF, ANP, Cardiodilatin, CDD, Cardiodilatin-related peptide, CDP, Prepronatriodilatin) is encoded by the Nppa gene (Gene ID: 24062) in rat. Atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP) are belong to the natriuretic peptide family. They are responsible for body fluid homeostasis and regulation of blood pressure. ANP is produced mainly by cardiac myocytes and is released in response to atrial stretch. Once in the circulation, ANP induces natriuresis, diuresis, and cause a reduction in blood pressure. In renal tissue ANP acts on receptors that increase glomerular filtration rate, reduce sodium reabsorption in the distal nephron, and diminish renin secretion. ANP has an intra-molecular ring structure connected by two cysteine residues and N-terminal and C-terminal extensions from it. ANP is produced with a signal peptide (aa 1-24) and a propeptide region (aa 25-122), which are cleaved to produce active hormone. It is cleaved by Corin by cleavage of Arg-Ser (aa 122-123) peptide bond. It can also be cleaved by membrane metalloendopeptidase between aa 129-130 that initiates its degradation and thereby regulate its activity.

Specificity

This rabbit polyclonal antibody detects Natriuretic peptides A in rat and murine heart. It targets an epitope within 28 amino acids from the C-terminal region.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal region of rat Natriuretic peptides A.

Application

Anti-Atrial Natriuretic peptide, Cat. No. AB5490-I, is a highly specific rabbit polyclonal antibody, that targets Natriuretic peptides A and has been tested in Western Blotting and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Atrial Natriuretic peptide in rat heart and mouse heart tissue sections.

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in rat heart tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Atrial Natriuretic peptide in rat heart tissue lysate

Target description

~17 kDa observed; 16.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat. packaging: antibody small pack of 25 . μ. L. manufacturer/tradename: Chemicon^®. . technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_006163.1. UniProt accession no.: P01161. target post-translational modification: unmodified. Gene Information: mouse ... Nppa(230899).