ANTI-BCL-11A CLONE 3B12 ZOOMAB RABBIT MONOCLONAL (C005B-262446)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

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Specificity

Clone 3B12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects B-cell Lymphoma/leukemia 11 (BCL-11A). It targets an epitope within 20 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human B-cell Lymphoma/leukemia 11 (BCL-11A).

Application

Quality Control Testing
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected BCL-11A in human tonsil tissue sections.Tested applicationsImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BCL-11A in HeLa and A431 cells.Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected BCL-11A in Ramos and Raji cell lysates.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-BCL-11A, clone 3B12 ZooMAb, Cat. No. ZRB1051, is a recombinant Rabbit monoclonal antibody that specifically targets BCL-11A and is tested for use in and Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

B-cell lymphoma/leukemia 11A (UniProt: Q9H165; also known as BCL-11A, B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, EVI-9, Zinc finger protein 856) is encoded by the BCL11A (also known as CTIP1, EVI9, KIAA1809, ZNF856) gene (Gene ID: 53335) in human. BCL-11A is a transcription factor associated with the BAF SWI/SNF chromatin remodeling complex that also acts as a repressor of fetal hemoglobin levels and plays a role in hematopoiesis. It is expressed at high levels in brain, spleen thymus, bone marrow and testis. Its expression has also been reported in CD34+ myeloid precursor cells, B-cells, monocytes and megakaryocytes. Its expression is tightly regulated during B-cell development. BCL-11A contains six C2H2 type zinc-finger regions. It is found in the nucleus as well as in the cytoplasm. In the absence of interaction between isoforms 1 and 2, it is predominantly localized in the cytoplasm and localizes to the nuclear paraspeckles when both isoforms are present. Chromosomal aberrations involving BCL11A gene are reported in B-cell malignancies. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.