ANTI-BCL-6 CLONE 1B7 ZOOMAB RABBIT MONOCLONAL (C15-1670-033)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B7 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects B-cell lymphoma 6 protein (BCL-6). It targets an epitope within the internal region.

Immunogen

His-tagged recombinant fragment corresponding to 242 amino acids from the internal region of human B-cell lymphoma 6 protein (BCL-6).

Application

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BCL-6 in Raji cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected BCL-6 in Ramos cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected BCL-6 in human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BCL-6 in A549 cells..

Affinity Binding Assay: A representative lot of this antibody bound BCL-6 with a KD of 1.8 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

B-cell lymphoma 6 protein (UniProt: P41182; also known as BCL-6, B-cell lymphoma 5 protein, BCL-5, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51) is encoded by the BCL6 (also known as BCL5, LAZ3, ZBTB27, ZNF51) gene (Gene ID: 604) in human. BCL-6 is a homodimeric protein with six C2H2 type zinc finger domains that is expressed in germinal center T- and B-cells and in primary immature dendritic cells. It acts as a transcriptional repressor that is mainly required for germinal cell (GC) formation. In GC B-cells, it represses genes that function in differentiation, inflammation, apoptosis, and cell cycle control. It allows GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunoglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. BCL-6 is also required for the establishment and maintenance of immunological memory for both T- and B-cells. Its levels are down-regulated during maturation of dendritic cells by selective stimuli such as bacterial lipopolysaccharides, CD40LG, and zymosan. BCL-6 levels are also shown to decline upon genotoxic stress in a dose- and time-dependent manner. Chromosomal aberrations involving BCL-6 are known to cause B-cell non-Hodgkin lymphomas. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Phan, RT., and Dalla-Favera, R. (2004). Nature. 432(7017); 635-639; Shaffer, AL., et al. (2000). Immunity. 13(2); 199-212).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.