General description

Dystrobrevin beta (UniProt O60941; also known as Beta-dystrobrevin, DTN-B) is encoded by the DTNB gene (Gene ID 1838) in human. Dystrobrevin alpha and beta are cytoplasmic components of dystrophin-associated glycoprotein complex (DGC) that links the extracellular matrix to the actin cytoskeleton. Whereas dystrobrevin alpha is ubiquitously expressed, dystrobrevin beta is expressed in non-muscle tissues, including brain. In addition to being a component of the DGCs, dystrobrevins also play roles in other functions via interact with various cellular proteins. A role of dystrobrevin beta in the regulation of chromatin dynamics is suggested based on its interaction with the high mobility group-domain proteins iBRAF and BRAF35. The function of dystrobrevins and their interactions with binding partners are regulated by post-translational modifications. Both PKA and PKC are shown to phosphorylate dystrobrevin beta on Thr11 that negatively impacts its binding affinity for the cargo-binding domainof kinesin heavy chain (Kif5A). Dystrobrevin shares sequence homology with the C-terminus of dystrophin, but does not bind dystroglycan. Dystrobrevin beta contains a ZZ-type zinc finger (a.a. 237-284), syntrophin-binding region (a.a. 399-448), a coiled-coil region (a.a. 428-529) that mediates the association between dystrobrevin and dystrophin.

Specificity

Clone 6B5.1 targeted epitope is present in human (spliced isoforms 1/3/4/6/7), mouse (isoforms 1 & 2), and rat beta-dystrobrevin reported by UniProt (O60941, O70585, P84060). Reactivity toward human spliced isoforms 2 and 5 that lack part of the immunogen sequence is possible, but has not been confirmed. Human isoforms 1, 2, and 3 are also known as DTN-B1, Dystrophin-associated protein A0, and DTN-B2, respectively.

Immunogen

GST-tagged recombinant internal fragment from the C-terminal half of human beta-dystrobrevin.

Application

Anti-Beta-Dystrobrevin, clone 6B5.1, Cat. No. MABN1611, is a highly specific mouse monoclonal antibody that targets beta-dystrobrevin and has been tested in Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected beta-dystrobrevin in 10 µg of mouse hypothalamus and rat hippocampus, as well as mouse and rat whole brain tissue lysates, but not in mouse cerebellum, human pituitary or whole brain tissue lysates.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected beta-dystrobrevin isoforms in 10 µg of human cerebral cortex tissue lysate.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this hybridoma culture supernatant detected beta-dystrobrevin in 10 µg of rat brain tissue lysate.

Target description

~58-90 kDa observed. 71.36/64.60/64.42/68.06/63.76/61.54/69.29 kDa (human isoform 1/2/3/4/5/6/7), 74.40/69.64 kDa (mouse isoform 1/2), 73.88 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Mouse IgG1 hybridoma culture supernatant without azide

Unpurified.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.