ANTI-B-GLUCURONIDASE (GUS)(N-TERMINAL) DEVELOPED IN RABBIT AFFINITY ISOLATED ANTIBODY

General description

β-Glucuronidase (GUS) is encoded by the E. coli gus gene (also referred to as uidA), GUS protein (60 kDa) is an hydrolase.

Specificity

Anti-β-Glucuronidase (N-Terminal) recognizes bacterial GUS expressed in transgenic tobacco plants.

Immunogen

Sythetic peptide corresponding to amino acids located at the N-terminus of E. coli GUS, conjugated to KLH.

Application

Anti-β-Glucuronidase (N-Terminal) antibody produced in rabbit has been used in western blotting.

Rabbit polyclonal anti-β-Glucuronidase (N-Terminal) antibody may be used for the detection of GUS by immunoblotting (60 kDa). It may be used to detect the reporter GUS marker protein in transgenic plants.

Biochem/physiol Actions

β-Glucuronidase (GUS) is a screenable markers E. coli GUS has been extensively used to monitor transgene delivery to plant tissue.

β-Glucuronidase catalyzes the cleavage of terminal glucuronic acid bound by β-linkage from mono-, oligo-, or polysaccharides or phenols. GUS is mainly used as a marker during transgenic events. In vitro GUS activity assays predominantly facilitates histochemical and fluorometric localization in tissues. GUS activity is also used for the analysis of expression of foreign genes. GUS reporter genes fused with specific promoter genes have been used for the determination of transformed tissues on culture media containing antibiotics. GUS-specific antibodies are helpful in detecting GUS gene product in transformed plants.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.