Anti-Beta III Tubulin Antibody, Alexa Fluor 488 Conjugate | AB15708A4 (C15-1315-820)

General description

Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.

Specificity

This antibody recognizes Beta III Tubulin.

Immunogen

Synthetic linear peptide corresponding to human Beta III Tubulin.

Application

Immunohistochemistry: Representive lot has been shown to work in adult mouse brain.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Quality

Evaluated by Immunocytochemistry in rat E18 cortex cells.

Immunocytochemistry Analysis: A 1:400 dilution of this antibody detected Beta III Tubulin in rat E18 cortex cells.

Target description

50 kDa calculated

Physical form

Affinity purified

Purified rabbit polyclonal conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat E18 cortex cells

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. conjugate: ALEXA FLUOR^™. 488. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse. species reactivity (predicted by homology): human (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable. NCBI accession no.: NP_006077. UniProt accession no.: Q13509. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.