ANTI-BETA-IV SPECTRIN CLONE N393/76

General description

Spectrin beta chain, non-erythrocytic 4 (UniProt: Q9H254; also known as Beta-IV spectrin, Spectrin, non-erythroid beta chain 3) is encoded by the SPTBN4 (also known as KIAA1642, SPTBN3) gene (Gene ID: 57731) in human. Beta-IV Spectrin belongs to the spectrin family of proteins and is abundantly expressed in brain and pancreatic islets. It belongs to the spectrin family of proteins and contains two CH (calponin-homology) domains, one PH domain, and 18 spectrin repeats. It has been isolated as an interactor of the receptor tyrosine phosphatase ICA512. Spectrin molecule is assembled as a tetramer of two alpha and two beta subunits. A tight association between the amino-terminal domain of an alpha subunit and the carboxyl-terminal domain of a beta subunit forms a heterodimer. In brain, beta-IV Spectrin co-localizes with ankyrin-G 480/270-kDa at initial segments and nodes of Ranvier of myelinated neurons. In brain its highest expression is observed in the cell bodies of large myelinated neurons. It is not detected in non-neuronal cells, including glial and endothelial cells.Beta-IV Spectrin along with ankyrin-G is required for the anchoring of voltage-gated Na+ channels and for cell adhesion molecule anchoring to the actin cytoskeleton. (Ref.: Berghs, S et al. (2000). J. Cell Biol. 151(5): 985-1002).

Specificity

Clone N393/76 specifically detects human and rat beta-IV Spectrin by immunohistochemistry. it targets a sequence within 192 amino acids in the C-terminal half of human beta-IV Spectrin.

Immunogen

A recombinant fusion fragment corresponding to 192 amino acids from the C-terminal half of human beta-IV spectrin.

Application

Anti-Beta-IV spectrin Antibody, clone N393/76, Cat. No. MABN1727, is a highly specific mouse monoclonal antibody that targets beta-IV spectrin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected beta-IV spectrin in human pancreas, human cervical cancer, and human uterus tissue.

Western Blotting Analysis: A representative lot detected beta-IV spectrin in COS cells transiently transfected with Myc-tagged beta 4-spectrin.

Immunohistochemistry Analysis: A representative lot detected beta-IV spectrin in rat cerebral cortex and hippocampus tissues.

Research Category
Neuroscience

Quality

Evaluated by Immunohistochemistry in human cerebellum tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected beta-IV spectrin in human cerebellum tissue.

Target description

288.98 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.