Anti-Bid Antibody (C15-1315-879)

General description

Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is known to interact with Bcl-2 and Bax. It has been reported that Bid induces cytochrome c release from mitochondria following cleavage by caspase-8 in response to apoptotic stimuli transmitted through cell surface death receptors. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Specificity

AB1735 recognizes Bid from human, mouse, rat and rabbit.

Immunogen

Recombinant full length GST-tagged mouse Bid protein

Application

Detect Bid using this Anti-Bid Antibody validated for use in WB, IP, IH.

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

Western blot: Recommended antibody dilution 1:3,000 to 1:6,000. Recognizes a band migrating at approximately 22 kDa corresponding to Bid.

Immunoprecipitation: Recommended antibody dilution 1:200

Immunohistochemistry: Recommended antibody dilution 1:1500

Optimal working dilutions must be determined by end user.

Linkage

Replaces: 04-438

Physical form

Whole serum without preservatives

Storage and Stability

Store at -20°C for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human, rat, rabbit. manufacturer/tradename: Chemicon^®. . technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NM_001196.2, NM_197966.1, NM_197967.1. UniProt accession no.: P55957. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... BID(637). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.