ANTI-BID CLONE 1G3 ZOOMAB« RABBIT MONOCLONAL (C15-1669-472)

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1G3 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Bid.

Immunogen

His-tagged, full-length, recombinant human Bid.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Bid in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:19,000 dilution from a representative lot detected Bid in lysate from MCF-7 cells and 1:1,000 dilution in lysates from C2C12, and PC12 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Bid in Human tonsil tissue sections.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Bid in Human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

BH3-interacting domain death agonist (UniProt: P55957; also known as p22 BID, BID) is encoded by the BID gene (Gene ID: 637) in human. Bid is a BH3 domain containing member of the Bcl-2 family  with proapoptotic function. It is shown to be crucial for death receptor-mediated apoptosis in many cell systems where it counters the protective effects of Bcl-2. It links the death receptor signaling pathway to the mitochondrial signaling pathway mediated by Bcl-2 proteins. Full length Bid that is localized in the cytoplasm is cleaved by caspase-8 to generate a truncated form (tBid) that translocates to mitochondria. tBid then binds to mitochondrial Bak and induces the release of cytochrome c. TNFα-induced caspase-mediated cleavage of Bid produced p15, 13, and p11 fragments.  Caspase-8-mediated Bid processing of Bid bridges the extrinsic and intrinsic apoptosis pathways that provide a mechanism to amplify death signals.  The BH3 motif of Bid is localized in amino acids 86-100. The hydrophobic FACE^® of the BH3 domain, which is exposed in other family members, is enclosed by the N-terminal of Bid and only exposed following proteolytic cleavage of Bid. A second functional domain in Bid known as the BH3B domain, which is localized in the N-terminus region, is reported to suppress the apoptogenic activity of the BH3 domain. The region between these two opposing domains is sensitive to proteolytic cleavage and contains cleavage sites for caspases and granzyme B. Four isoforms produced by Alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Renshaw, SA.,  et al. (2004). J. Biol. Chem. 279(4); 2846-2855; Esposti, MD. (2002). Apoptosis. 7; 433-440).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Affinity is a trademark of Mine Safety Appliances Co.

FACE is a registered trademark of Glyko Inc.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.