General description

Brefeldin A-inhibited guanine nucleotide-exchange protein 2 (UniProt: Q9Y6D5; also known as Brefeldin A-inhibited GEP 2, ADP-ribosylation factor guanine nucleotide-exchange factor 2, BIG2) is encoded by the ARFGEF2 (also known as ARFGEP2, BIG2) gene (Gene ID: 10564) in human. BIG1 and BIG2, are important activators of ADP-ribosylation factors for vesicular trafficking. BIG1 and BIG2 display about 74% homology in their amino acid sequence and about 90% identity in their Sec7 domains. BIG2, contains a 200-aa Sec7 domain that is responsible for this catalytic activity and its inhibition by brefeldin A. The Sec7 domain is located near the center of the molecule and serves to accelerate replacement of GDP bound to ARF with GTP. BIG2 is expressed in placenta, lung, heart, brain, kidney and pancreas. BIG2 is shown to promote guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. It promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. BIG2 is required for the integrity of the endosomal compartment and is involved in trafficking from the trans-Golgi network (TGN) to endosomes. In vitro phosphorylation of BIG2 by PKA reduces its GEF activity. Mutations in ARFGEP2 gene are known to cause periventricular nodular heterotopia 2 (PVNH2), which is characterized by the presence of periventricular nodules of cerebral gray matter, resulting from a failure of neurons to migrate normally from the lateral ventricular proliferative zone, where they are formed, to the cerebral cortex. PVNH2 causes developmental delays and makes the subject to recurrent infections. (Ref.: Li, H et al. (2003). Proc. Natl. Acad. Sci. USA 100(4):1627-32).

Specificity

Clone 56 specifically detects BIG2 in human. It targets an epitope within amino acids 9-257 in the N-terminal region.

Immunogen

GST-tagged recombinant fragment corresponding to 249 amino acids from the N-terminal region of human Brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2).

Application

Detect BIG2 using this mouse monoclonal Anti-BIG2 Antibody, clone 56, Cat. No. MABS1246. It has been tested in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 2 µg/mL from a representative lot detected BIG2 in 10 µg of HeLa cell lysate.

Immunoprecipitation Analysis: A representative lot detected BIG2 in Immunoprecipitation applications (Lowery, J., et. al. (2013). J Biol Chem. 288(16):11532-45).

Immunocytochemistry Analysis: A representative lot detected BIG2 in Immunocytochemistry applications (Lowery, J., et. al. (2013). J Biol Chem. 288(16):11532-45).

Western Blotting Analysis: A representative lot detected BIG2 in Western Blotting applications (Lowery, J., et. al. (2013). J Biol Chem. 288(16):11532-45).

Quality

Evaluated by Western Blotting in human brain tissue lysates.

Western Blotting Analysis: 2 µg/mL of this antibody detected BIG2 in 10 µg of human brain tissue lysates.

Target description

~202 kDa observed; 202.04 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.