ANTI-BIN1 CLONE 99D ALEXA FLUOR« 488 CONJUGATE

General description

Myc box-dependent-interacting protein 1 (UniProt: O00499; also known as Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1, BIN1) is encoded by the BIN1 (also known as AMPHL) gene (Gene ID: 274) in human. BIN1 is expressed in many tissues, but has the highest expression in brain and muscle. In brain it is concentrated in the cytomatrix beneath the plasma membrane of axon initial segments and nodes of Ranvier. In skeletal muscle, it is concentrated around the plasmalemma of T tubules. BIN1 is involved in regulation of synaptic vesicle endocytosis and is also reported to act as a tumor suppressor that inhibits malignant cell transformation. BIN1 is considered as a dominant autoantigen in paraneoplastic Stiff-man syndrome. Mutations in BIN1 gene are known to cause myopathy centronuclear 2 (CNM2), a congenital muscle disorder characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. Ref.: Butler, MH., et al., (1997). J. Cell Biol. 137(6): 1355 1367.

Specificity

Clone 99D, Alexa Fluor^™ 488 Conjugate detects Bin1 in A431 and HeLa cells by immunocytochemistry.

Immunogen

Linear peptide corresponding to human Bin1.

Application

Detect Bin1 using this mouse monoclonal Anti-Bin1 antibody, clone 99D, Alexa Fluor^™ 488 Conjugate, Cat. No. 05-449-AF488, validated for use in Flow Cytometry and Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bin1 in HeLa cells.

Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected Bin1 in one million HeLa cells.

Research Category
Apoptosis & Cancer

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bin1 in A431 cells.

Target description

64.7 kDa calculated.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG2b in PBS with 1.5% BSA and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.