ANTI-BLUE CLONE 2D2-F11 HRP CONJUGATED (C005B-451687)

General description

Platelet-derived growth factor subunit A (UniProt: P04085d; also known as PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide) is encoded by the PDGFA (also known as PDGF1) gene (Gene ID: 5154) in human. PDGFs are a family of four cystine-knot-type growth factors (PDGF-A, -B, -C and -D) that are dimeric mitogenic signaling molecules. In the early developmental stage, they drive the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages they participate in tissue remodeling, cellular differentiation, and morphogenesis. PDGF A and B can exist as two A subunits (PDGF-AA), two B subunits (PDGF-BB), or one of each (PDGF-AB). These dimeric forms are differentially expressed in various cell types. Their action is mediated via two types of receptors PDGFR and PDGFRβ, which belong to the class III receptor tyrosine kinases and exhibit different expression patterns and physiological roles. PDGFR signaling controls gastrulation and the development of several organs such as lung, intestine, skin, testis, kidney, bones, and neuroprotective tissues and PDGFRβ signaling is an essential regulator of early hematopoiesis and blood vessel formation. PDGF-A is homodimeric protein that is synthesized with a signal peptide (aa 1-20) and a propeptide (aa 21-86) that are subsequently cleaved off to produce the mature protein (aa 87-211). Its signaling activity is modulated by the formation of heterodimers with PDGFB. It plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival, and chemotaxis. It is also required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. It is also involved in normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum.

Specificity

Clone 5C10.8 is a mouse monoclonal antibody that specifically detects Platelet derived growth factor AA (PDGF-AA).

Immunogen

Recombinant full-length human Platelet derived growth factor A (without the signal peptide and propeptide).

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant human PDGF-AA.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant human PDGF-AA.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected PDGF-AA in human testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-PDGF-AA, clone 5C10.8, Cat. No. MABC1701, is a mouse monoclonal antibody that detects PDGF-AA and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2b in PBS with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.