Anti-Brain Derived Neurotrophic Factor Antibody (C15-1315-758)

Specificity

Recognizes Brain Derived Neurotrophic Factor (BDNF). By dot blot, less than 0.1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4.

Immunogen

A 10 amino acid peptide corresponding to the amino-terminal of mouse Brain Derived Neurotrophic Factor (BDNF) coupled to ovalbumin. The sequence is H₂N-HSDPARRGEL-COOH.

Application

Anti-Brain Derived Neurotrophic Factor Antibody is an antibody against Brain Derived Neurotrophic Factor for use in ELISA, IH & WB.

Immunohistochemistry: 1-10 μg/mL (see suggested protocol).

Immunoblotting: 1-10 μg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as 18 kDa, with a homodimer at ~27-30 kDa.

ELISA (one-site): 1-10 μg/mL

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

Neuroinflammation & Pain

Target description

18 kDa (monomer) and 27-30 kDa (homodimer)

Physical form

Format: Purified

Protein A Purified immunoglobulin fraction lyophilized from PBS, with no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).

Protein A purified

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, rat, human. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_001709.3, NM_170731.3, NM_170732.3, NM_170733.2, NM_170734.2, NM_170735.4. UniProt accession no.: P23560. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... BDNF(627). Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.