ANTI-C-ERBB2/C-NEU (AB-4) MOUSE MAB (7.16.4)

General description

Anti-c-ErbB2/c-Neu (Ab-4), mouse monoclonal, clone 7.16.4, recognizes ~185 kDa c-ErbB2 in overexpressing B104-1-1 cells. It is validated for WB, IP, ICC, frozen sections, & growth inhibition assay.

Purified mouse monoclonal antibody generated by immunizing C3H mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~185 kDa c-ErbB2/c-neu protein.

Recognizes the ~185 kDa c-ErbB2/c-Neu protein in overexpressing B104-1-1 cells.

Immunogen

B104-1-1 cells overexpressing rat c-ErbB2/c-Neu

Epitope: Within the extracellular domain

Human

Application

Frozen Sections (5 g/ml, see application references)
Immunoblotting (see application references)
Immunocytochemistry (5 g/ml)
Immunoprecipitation (1 g/ml, see application references)
Neutralization Studies (not recommended, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Negative Control
Fisher rat embryo cells

Positive Control
B104-1-1 cells

Other Notes

Stains frozen tissue sections of rat cells overexpressing c-neu. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.

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Schwab, M., et al. 1984. Cold Spring Harbor Laboratory2, 215.

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