General description

c-Fos belongs to a family of transcription factors, including FosB, Fra1, Fra2. The fos oncogene was isolated as a retroviral transforming gene (v-fos) carried by the FBJ-murine osteosarcoma virus. c-Fos undergoes post-translational modifications and other forms have been described including 57 Da, 60 kDa and 62 kDa proteins. Stable expression of c-Fos has been shown in developing bone tissue and teeth, hematopoietic cells,germ cells, and in the central nervous system. High levels of c-Fos are expressed in human full term fetal membranes.

Immunogen

synthetic peptide corresponding to the N-terminal region of the human c-Fos proto-oncogene (p55), amino acids 3-16.

Application

Rabbit polyclonal anti-c-Fos antibody recognizes c-Fos by immunoblotting, (as single or multiple bands at 50-62 kDa) and by immunohistochemistry. By immunoblotting, the antibody may also detect bands of ~40 kDa representing c-Fos degradation products. Staining of c-Fos by immunoblotting is specifically inhibited with c-Fos immunizing peptide (c-Fos human, amino acids 3-16 with C-terminally added lysine).
Anti-c-Fos antibody produced in rabbit has been used in immunoassays to determine the c-fos immunoreactivity in spinal cord sections. It has been used in Immunohistochemistry.
It may be used as a probe to determine the presence and roles of c-Fos in a wide variety of cell processes including cell proliferation and differentiation, and responses to cell damage or stress.

Biochem/physiol Actions

c-Fos (AP-1 transcription factor subunit) plays an important role in cell proliferation and differentiation. In addition, it is involved in cellular responses to stress, cell damage and death, and has a central role in normal bone and hematopoietic cell development and in oncogenesis. c-Fos is rapidly and transiently induced in almost every cell type upon stimulation by a variety of extracellular stimuli, including stress, mitogenic growth factors, cytokines, neurotransmitters and pharmacological agents. Increased expression of c-Fos is found in some human carcinomas, including colon and pancreatic adenocarcinoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

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