ANTI-C-K-RAS ANTIBODY CLONE 234-4.2

General description

GTPase KRas (c-k-ras; K-ras) is a membrane-bound G protein belonging to the family of Ras GTPases. They are activated by the binding of GTP in response to signals from various receptors including the epidermal growth factor receptor (EGFR). Ras GTPases are regulated by guanine nucleotide exchange factors (GEF) and GTPase activating proteins (GAP) and lie upstream of the MAP kinase pathway which controls a range of cellular processes including proliferation and differentiation. Previous studies have suggested that KRas plays an important role in embryonic development, and it may also contribute to the pathogenesis of colorectal cancer via an EGFR-mediated pathway.

Immunogen

Recombinant protein corresponding to rat c-K-Ras.

Application

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

This Anti-c-K-Ras Antibody, clone 234-4.2 is validated for use in Western Blotting, IHC for the detection of c-K-Ras.

Western Blot Analysis: A representative lot detected c-K-Ras in A431 cell lysate.

Western Blot Analysis: A representative lot from an independent laboratory detected c-K-Ras in primary human
lymphoid leukemia cell lysates, and in normal and HA-dynamin K44A mutant HeLa cell lysates (Omerovic, J., et al. (2008). Oncogene. 27(19):2754-2762.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected c-K-Ras in non-small cell lung cancer cells (Volm, M., et al. (2002). Clin Cancer Res. 8(6):1843-1848.).

Quality

Evaluated by Western Blot in SW-480 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected c-K-Ras in 10 µg of SW-480 cell lysate.

Target description

~22 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
SW-480 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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