ANTI-C1ORF43 (C15-1447-440)

General description

C1orf43 (chromosome 1 open reading frame 43) belongs to epidermal differentiation complex (EDC) family of genes. It is mapped to human chromosome 1q21, and has three cDNA variants owing to alternative splicing. It might have the structural characteristics of N-4 cytosine-specific DNA methylases, and is expressed in multiple tissues. It is also found in CD34⁺ hematopoietic stem cells (HSPCs). C1orf43 gene shares no homology with any of the existing EDC families.

Immunogen

Uncharacterized protein C1orf43 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

EDC (epidermal differentiation complex) family of genes is responsible for the normal maturation of epidermis and is therefore, linked with skin diseases. EDC is also associated with carcinogenesis, and the expression of C1orf43 is found to be up-regulated in human hepatocellular carcinoma (HCC). Increased expression of this gene in Focus and WRL-68 HCC cell lines leads to cell proliferation and colony formation. Studies also suggest that this protein is induced by NS5A (non-structural protein 5A) present in hepatitis C virus (HCV). Therefore, C1orf43 might lead to HCV-related HCC. Fibrolamellar carcinomas (FLC), a rare form of HCC, also shows increased levels of C1orf43 expression. Hence, C1orf43 might be an oncogene for HCC. The up-regulation of this gene can also act as a marker for poor prognosis, and determination of malignance in breast cancer.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST72033.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.