ANTI-CALCIUM CHANNEL (PAN A1 SUBUNIT) DEVELOPED IN RABBIT AFFINITY ISOLATED ANTIBODY

Specificity

The epitope recognized by this antibody is masked by α2 and δ chains; therefore, the antibody binds only after subunit dissociation using Triton X-100 or other detergents.

Immunogen

synthetic peptide corresponding to amino acids 1506-1524 of the α_1S subunit of rat skeletal muscle voltage-gated calcium channel (VGCC, CP15) (Accession P22002) with additional N-terminal cysteine, conjugated to MBS-KLH.

Application

Anti-Calcium Channel (α1 Subunit), Pan antibody produced in rabbit is suitable for immunohistochemistry (using rat heart sections) and western blotting at a dilution of 1:200 using rat brain membranes.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Biochem/physiol Actions

Calcium channel, voltage-dependent, L type, α1C subunit (Ca_v1.2) is a protein encoded by the CACNA1C gene in humans and belongs to the family of voltage-gated calcium channels. It serves as the key transducers of cell surface membrane potential changes into local intracellular calcium transients that initiate different physiological events. CACNA1C is a subunit of L-type voltage-dependent calcium channel. It is mapped to chromosome 12p13.3 and is implicated as a susceptibility gene for schizophrenia. CACNA1C is selectively expressed in human TH2 cells and blockage of Ca_v1.2 channel activation in TH2 cells may represent new strategies to treat allergic diseases in human subjects. Its increased activity is implicated in the pathogenesis of dementia and Alzheimer′s disease (AD).

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, 5% sucrose and 0.025% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.