Anti-Calcium Channel CaV3.2 (alpha1H) antibody produced in rabbit

General description

Calcium channel, voltage-dependent, T type, α1H subunit (CACNA1H) is a protein encoded by the CACNA1H gene in humans and is mapped to chromosome 16p13.3. The proteins are membrane protein machinery performing selective permeation of external calcium ions. The α1H channel is sensitive to mibefradil, a nondihydropyridine Ca²⁺ channel blocker. CACNA1H is abundantly expressed in both peripheral and central endings of the primary afferent neurons, regulating neuronal excitability and release of excitatory neurotransmitters.

Immunogen

synthetic peptide CHVEGPQERARVAHS corresponding to amino acid residues 581-595 of rat Ca_V3.2. Epitope location: intracellular loop connecting D1-D2. Mouse, bovine, and canine have 14/15 residues identical; human has 13/15 residues identical.

Application

Anti-Calcium Channel Ca_V3.2 (α1H) antibody produced in rabbit is suitable for western blotting at a dilution of 1:200 using lysate from the ND7/23 cell line.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Calcium channel, voltage-dependent, T type, α1H subunit (CACNA1H) plays an essential role in regulating neuronal excitability and network oscillations in the brain. Mutations in the gene are associated with various forms of idiopathic generalized epilepsy. Gaining-of-function mutation in CACNA1H increases seizure susceptibility by directly altering neuronal electrical properties and indirectly by changing gene expression. CACNA1H acts as an important mediator of Ca²⁺ entry near the resting membrane potential. It plays a pivotal role in processing of pain signals and may serve as a novel target for development of drugs for the treatment of intractable pain resistant to currently available analgesics. CACNA1H up-regulation is involved in the pathophysiology of inflammatory, neuropathic and visceral pain.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.