General description
Calmodulin-like protein 5 (UniProt Q9NZT1; also known as Calmodulin-like skin protein) is encoded by the CALML5 (also known as CLSP) gene (Gene ID 51806) in human. Epidermal differentiation and barrier function depend on many calcium-regulated proteins and enzymes. CALML5/CLSP is expressed in the stratum granulosum and lower layers of the stratum corneum of the skin, where it modulates the function of calcium-dependent proteins, such as transglutaminase 3. CALML5/CLSP level is upregulated in granular and spinal keratinocytes during the later stages of wound healing in human epidermis and eventually returns to basal level once the wound is closed and barrier function has been restored. In the hyperproliferative skin disorders psoriasis and atopic dermatitis (AD), the permeability barrier is disturbed, causing an increased CLSP built-up as a result of decresed CLSP degradation due to an altered calcium gradient.
Specificity
Clone 3F6.1 recognizes an epitope outside the first EF-hand motif.
Immunogen
Epitope: Outside the first EF-hand motif.
GST-tagged recombinant human CALML5 lacking the first EF-hand motif.
Application
Anti-CALML5 Antibody, clone 3F6.1 is an antibody against CALM5 for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected CALML5 in human tonsil and skin tissue sections.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Quality
Evaluated by Western Blotting of human CALML5 GST fusion protein.
Western Blotting Analysis: 0.05 µg/mL of this antibody detected 0.05 µg of human CALML5 GST fusion protein.
Target description
15.89 kDa calculated. Uncharacterized band(s) may appear in some lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 51261502
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABN1583
- Temperature Control Device:
- Yes
