General description

Anti-Calretinin is developed in rabbit using a synthetic peptide corresponding to the C-terminal region of rat calretinin (amino acids 194-209) conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Calretinin is an intracellular Ca²⁺-binding protein, with broad tissue distribution and is a member of the calmodulin superfamily. It is expressed in certain populations of neurons in the central and peripheral nervous systems. It is abundant in the cerebellum (granule cells), olfactory bulb and auditory pathways.

Immunogen

synthetic peptide corresponding to the C-terminal region of rat calretinin (amino acids 194-209) conjugated to KLH. This sequence is specific for calretinin and not found in other members of the EF-hand family such as calbindin-D-28K, calbindin-D-9K, myosin light chain, parvalbumin, S-100a, S-100b, S100A2 (S100L) and S100A6 (calcyclin). This sequence is identical in human and mouse calretinin and highly conserved in chicken calretinin.

Application

Anti-Calretinin antibody produced in rabbit has been used in immunohistochemistry and immunofluorescence.

Anti-Calretinin antibody produced in rabbit is suitable for the following applications:

immunohistochemistry
microarray
western blotting at a dilution of 1:1,000 using recombinant human calretinin

Biochem/physiol Actions

The physiological function of calretinin is not known, but it seems to be related to Ca²⁺ buffering and diffusion. Calretinin-deficient mice show impaired long-term potentiation (LTP) in the dentate gyrus, impaired motor coordination and Purkinje cell excitability. A role for both calretinin and calbindin-D-28K in synaptic plasticity and in neuroprotection from excitotoxic insults has been postulated. By acting as a buffer or sensor of intracellular free Ca²⁺, calretinin promotes calcium homeostasis. Furthermore, several truncated forms of calretinin have been found in certain tumor cells, but are absent from normal cells, suggesting that calretinin may play a role in tumorigenesis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.