General description

Calsequestrin is a major Ca²⁺ binding protein in cardiac and skeletal muscle.

Calsequestrin-2 (55 kDa) is found in both sarcoplasmic reticulum′s (SR) terminal cisternae luminal space of both cardiac and in slow skeletal muscle cells. It is made of three domains each with negatively charged thioredoxin-like folds with a β-sheet surrounded by four α-helices.

Immunogen

synthetic peptide corresponding to amino acids 57-74 of rat calsequestrin-2, conjugated to KLH. This sequence is highly conserved in human and rat calsequestrin-2 (single amino acid substitution). This sequence is not found in calsequestrin-1.

Application

Anti-Calsequestrin-2 antibody has been used in immunoblotting, western blotting.

Anti-Calsequestrin-2 is suitable for western blot at a concentration of 0.5-1.0μg/mL using HEK-293T cells expressing human calsequestrin-2 and western blot at a concentration of 0.5-1.0μg/mL using extract of rat cardiac muscle (S1 fraction)

Biochem/physiol Actions

Over expression of cardiac calsequestrin (CASQ2) is associated with depressed cardiovascular function and hypertrophy. Mutations in CASQ2 are thought to be associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). It is a high-capacity, low-affinity Ca²⁺ binding glycoprotein, which functions as an internal Ca²⁺ store in the lumen of the sarcoplasmic reticulum (SR).

Physical form

Solution in 0.01 M phosÂphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

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