General description
CB1 is a protein that is one of two cannabinoid receptors (CB1 CB2). Cannabinoid principally delta-9-tetrahydrocannabinol (THC) and its synthetic analogs, are the active psychoactive compounds present is marijuana. These cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptors which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Three splice variants have been found for this gene. CB1 is primarily located in brain, and CB2 is found macrophages.
Specificity
Recognizes rat Cannabinoid Receptor 1 (CB1). The immunogen shows no significant sequence homology with CB2 or other receptors.
SPECIES REACTIVITIES: The immunogen peptide is 100% conserved mouse, 94% in monkey and human, 88% in zebra finch, 75% in feline and newt, (salamander) and 73% in puffer fish. Reactivity with other species has not been confirmed.
Immunogen
A 20 amino acid peptide sequence near the cytoplasmic, C-terminus of rat CB1 (Gerard et al. 1991; Gerard et al.
1990; Shire et al. 1995; Matsuda et al. 1990; Ho et al. 1996; Chakraborty et al. 1995).
Epitope: C-terminus
Application
Anti-Cannabinoid Receptor 1 Antibody, C-terminus is an antibody against Cannabinoid Receptor 1 for use in ELISA & WB.
Research Category
Neuroscience
Research Sub Category
Neuroinflammation & Pain
Neurotransmitters & Receptors
Western blot: 1-10 μg/mL using ECL. Expected sizes are ~60kDa and 80-84kDa under reducing conditions.
Immunohistochemistry: not tested. It is recommended that the antibody be tried at 2-10 μg/mL on paraformaldehyde fixed tissue.
ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide (Catalog number AG213) per well.
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Liquid in phosphate buffered saline containing 0.1% BSA.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, mouse. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, western blot: suitable. NCBI accession no.: NM_016083.3, NM_033181.2. UniProt accession no.: P21554. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: mouse ... Cnr1(12801). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51131627
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB5638P