Anti-CAPRIN1 antibody produced in rabbit (C15-1448-934)

General description

Cell cycle associated protein 1 (CAPRIN1) gene is mapped to human chromosome 11p13. The gene codes for highly conserved cytoplasmic phosphoprotein. CAPRIN1 belongs to the conserved caprin family. The protein is ubiquitously expressed and is characterized with two highly conserved regions, homologous region-1 and −2, arginine-glycine-glycine (RGG) motif and the RG enrichment region.

Immunogen

Caprin-1 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Anti-CAPRIN1 antibody produced in rabbit has been used in immunohistochemistry (IHC) and western blotting.

Biochem/physiol Actions

Cell cycle associated protein 1 (CAPRIN1) is associated with cell proliferation and is essential for normal advancement through the G₁-S phase of the cell cycle. It is involved in the formation of stress granules via phosphorylation of eIF2α (a transcription initiation factor) and also regulates the translation of mRNAs of proteins related to synaptic plasticity in neurons. Studies have shown that Japanese encephalitis virus (JEV) causes a translational shutoff by preventing the formation of stress granules through interaction of JEV core protein with CAPRIN1. It has also been shown that it may be involved in the development of osteosarcoma.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST72806.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.