General description
Carboxypeptidase M (UniProt: D4A9Q5; also known as CPM) is encoded by the Cpm gene (Gene ID: 314855) in rat. Rat CPM a glycosylphosphatidylinositol (GPI) anchored metallo-carboxypeptidase that cleaves basic carboxyl-terminal amino acids from various peptides at neutral pH. Rat CPM is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to generate the mature form. The mature CPM displays high specificity and cleaves only C-terminal arginine or lysine residues in a variety of peptide substrates. It is shown to be expressed in the lung and placenta at high levels. Its expression is detected in alveolar cells lacking T1alpha, a type I cell marker protein found in adult lung. Carboxypeptidase M also serves as a macrophage differentiation marker, which is preferentially induced in epithelioid cells of all granuloma types studied, but not in resting macrophages. Hence, it can be employed for detection of minute granuloma even in the presence of non-granulomatous macrophages. Clone 7F9 recognizes CPM expressed in late embryonic rat lung to postnatal day 1. (Ref.: Fujiwara, N., et al. (2007), Respirology 12(1); 54-62; Tsakiris, I., et al. (2012). Lab. Invest. 92(3); 345-361).
Specificity
Clone 7F9 specifically detects carboxypeptidase M in rat tissues.
Immunogen
Epitope: extracellular domain
Membrane fraction of E18.5 embryonic rat lung.
Application
Anti-carboxypeptidase M, clone 7F9, Cat. No. MABS1925, is a mouse monoclonal antibody that detects Carboxypeptidase M and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A representative lot detected carboxypeptidase M in Western Blotting applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).
Immunoprecipitation Analysis: A representative lot immunoprecipitated carboxypeptidase M in Immunoprecipitation applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).
Immunohistochemistry Analysis: A representative lot detected carboxypeptidase M in Immunohistochemistry applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).
Quality
Evaluated by Immunofluorescence in E19 rat embryo tissue sections.
Immunofluorescence Analysis: A 1:25 dilution of this antibody detected carboxypeptidase M in E19 rat embryo tissue sections.
Target description
50.32 kDa calculated.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- UPC:
- 12352209
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABS1925-25UG
