General description
Caspase-1 (ICE, interleukin-1b-converting enzyme) is the mammalian caspase responsible for the proteolytic conversion of the proforms of interleukin-1b and IL-18 into active cytokines. Like other caspases, caspase-1 itself is synthesized as a proenzyme that is cleaved during activation into large (20 kDa) and small (10 kDa) subunits.
Specificity
AB1871 recognizes the 45 kDa procaspase-1 as well as the 20 kDa cleaved caspase-1.
Application
This Anti-Caspase 1 Antibody is validated for use in IF, IP, WB for the detection of Caspase 1.
Western blot: 1-4 μg/mL
Immunoprecipitation: 4-8 μg/mL
Immunofluorescence: 10-20 μg/mL
Optimal working dilutions must be determined by end user.
Physical form
Affinity purified immunoglobulin. Liquid in PBS, pH 7.2, containing 50% glycerol, 1% BSA and 0.02% sodium azide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, rat, mouse. manufacturer/tradename: Chemicon®. . technique(s): immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NM_001223.3, NM_033292.2, NM_033293.2, NM_033294.2, NM_033295.2. UniProt accession no.: P29466. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CASP1(834)mouse ... Casp1(12362)
rat ... Casp1(25166). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.
- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB1871
